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KMID : 0545120060160091448
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Journal of Microbiology and Biotechnology
2006 Volume.16 No. 9 p.1448 ~ p.1452
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Influence of Gluconeogenic Phosphoenolpyruvate Carboxykinase (PCK) Expression on Succinic Acid Fermentation in Escherichia coli Under High Bicarbonate Condition
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Kwon Yeong-Deok
Lee Sang-Yup
Kim Pil
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Abstract
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The effects of amplifying the gluconeogenic phosphoenolpyruvate carboxykinase of Escherichia coli (pckEc) on succinic acid production in E. coli were examined under anaerobic condition. No significant increase in succinic acid production was observed in E. coli overexpressing the pckEc gene without supplementing NaHCO3 or MgCO3. On the other hand, succinic acid production was enhanced as the NaHCO3 concentration was increased. When 20 g/l of NaHCO3 was added, succinic acid production in recombinant E. coli overexpressing PCK was 2.2-fold higher than that observed in the wild-type strain. It was concluded that the gluconeogenic pckEc overexpression enabled E. coli to enhance succinic acid production only under the high bicarbonate supplementation condition.
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KEYWORD
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Escherichia coli, succinic acid, phosphoenol phosphoenolpyruvate, NaHCO3
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